Skills

Developmental and stem cell biology:

• Isolation and culture of embryonic stem (ES) cells and mesenchymal stem cells from mouse and human,
• Establishment of induced pluripotent stem cells (iPSCs) from mouse and human cells.
• Establishment of human patient’s iPSC lines from patients with Spina Bifida and Atrial Fibrillation.
• Establishment of iPSC- disease models from patient’s somatic cells.
• Differentiation of ESs or iPSCs into adult cells, for example, neural differentiation, cardiac differentiation, endothelial cells etc.
• Somatic cell nuclear transfer, Microinjection, Sperm injection, Embryo chimera, Embryo transfer.

Molecular and cellular biology:

• Isolation and purification of DNA, RNA, micro RNA and protein from cells and tissues.
• Electrophoresis.
• PCR, Quantity PCR(qPCR),
• Northern blot, electrophoretic mobility shift assay (EMSA), immunoprecipitation (IP), co-IP.
• Plasmid construction, Plasmid transfection, Gene expression in cells.
• Isolation and maintenance of cells including blood cells, lymphocytes, fibroblast, cardiac myocytes, endothelial cell, tumor cells, etc.
• Gene knock-out, knock-in and knock-down.

Immunology and histochemistry:

• Immunoblotting,
• Antibody preparation,
• Western blot,
• immunofluorescence,
• Fluorescence-activated cell sorting (FACS).
• Paraffin section, frozen section, histochemistry staining.

Others 

• Standard techniques of immunology, microbiology, cell biology, biochemistry and molecular biology.
• Office software including word, powerpoint.
• Data collection.
• data analysis.
• Web design, HTML, CSS, BOOTSTRAP, JAVASCRIPT.
• Python, java. SAS.